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Synopses of innovative studies using nonculture-based procedures Study 1 Goal: Ask Who is there A comparative study. Strategy: Sample several colonic mucosal sites and feces of three healthy humans (total number of samples processed = 21). Method: Extract nucleic acids. Use PCR to amplify 16S rRNA genes. Clone and sequence the biomarker genes (see Section 6.7). Results: 13,355 prokaryotic 16S rRNA gene sequences from two broad sources in the large intestine of three people. Message: 11,831 bacterial 16S rRNA gene sequences and 1524 archael 16S rRNA gene sequences; the most extensive survey to date. (A) The habitat is highly selective: (i) >99% of the bacterial sequences fell within only two taxonomic divisions (Bacteriodetes and Firmicutes); and (ii) all archaeal sequences belonged to a single species (Methanobrevibacter smithii). (B) The majority of sequences were novel, corresponding to uncultivated microorganisms. (C) Signi cant variability in community composition was found between human subjects and sampling sites within a given subject. Study 2 Goal: Ask What genes are there by performing metagenomic functional analysis (see Sections 3.2 and 6.9). Strategy: Sample fecal DNA from two healthy individuals. Create metagenomic DNA libraries. Method: Use shotgun sequencing procedures to attempt to clone and sequence all genes. Also use PCR to amplify, clone, and sequence 16S rRNA genes. Results: ~78 106 bp of DNA were sequenced and 2062 PCR-ampli ed 16S rRNA genes were examined. COG (clusters of orthologous groups) and KEGG (Kyoto encyclopedia of genes and genomes) approaches were used to assess gene function (see Section 3.2). Message: (A) Both shotgun cloning and PCR ampli cation of 16S rRNA genes revealed a dominance of Firmicutes-related taxa. Bacteriodetes were largely absent. (B) Shotgun sequencing of functional genes revealed enriched genes for polysaccharide hydrolysis and uptake, amino acid synthesis, vitamin synthesis, and methanogenesis.

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If you need to create several different printed reports from the same Excel workbook, setting up the specific settings for each report can be a tedious job. For example, you may need to print a full report in landscape mode for your boss. Another department may require a simplified report using the same data, but with some hidden columns in portrait mode. You can simplify the process by creating custom named views of your worksheets that include the proper settings for each report. The Custom View feature enables you to give names to various views of your worksheet, and you can quickly switch among these named views. A view includes settings for the following: n Print settings, as specified in the Page Layout Page Setup, Page Layout Scale To Fit, and Page Page Setup Sheet Options groups. n Hidden rows and columns n The worksheet view (Normal, Page Layout View, Page Break Preview) n Selected cells and ranges n The active cell n The zoom factor n Window sizes and positions n Frozen panes If you find that you re constantly fiddling with these settings and then changing them back, using named views can save you lots of effort.

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FIGURE 11-4

The value in Char Width determines the visible width of the field; the value in Max Chars actually determines the number of characters that can be entered.

4. Choose the Start button. Dreamweaver processes the entire site for Library updates. Any Library items contained are modified to reflect the changes.

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You can create lines of any shape and size and then change the style, width, and color. Here, I m adding two arrowheads to the document s master page so that it appears on all pages. To work on a master page, double-click a master page icon in the Pages panel (choose Window Pages or press +F12 or Ctrl+F12).

14

Compiling and organizing a component library made of both pre-made and custom components (Fig. 3-24) is important. Organize your components by category types (for example, trees, benches, buildings). The Components menu (Window > Components) can be linked to a user component library for easy access (Fig. 3-25).

Including Row Numbers Adjusting the Column Width Preserving Column Sort/Filter/Layout Preserving Formatting Changes

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Tip If you can t see the outline of the form, choose View Visual Aids Invisible Elements. If you still can t see the form, choose Edit Preferences (Dreamweaver Preferences) and select the Form Delimiter checkbox in the Invisible Elements category. Clear the checkbox if you don t want to see the form outline.

However, you are not restricted to working with the default HSB model. If you prefer another model, you can alter the display to suit your workflow. For instance, you can set it so that it works on the RGB color model or the Lab color model. The radio buttons to the left of the text fields define which model and how that model affects the behavior of the slider and the color field (Figures 2-27 and 2-28).

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